Description
Key facts
Detection method:Fluorescent,
Sample types:Tissue Lysate, Saliva, Urine, Plasma, Serum, Cell Lysate,
Assay type:Quantitative,
Sensitivity:= 1 µM,
Assay Platform:Microplate reader
Product details:
Glycine Assay Kit (Fluorometric) (ab211100) provides a provides a simple and sensitive assay that detects physiological concentrations of glycine (GLY).
Glycine assay principle
In the Glycine assay, Glycine is oxidized in the presence of GLY Enzyme Mix and is converted to an intermediate, which reacts with a fluorescent probe to generate a strong stable signal at Ex/Em = 535/587 nm. The intensity of the signal is directly proportional to the amount of GLY in the sample.
The reaction is specific and other amino acids do not interfere with the assay.
Glycine assay protocol summary
- Add samples and standards to wells
- Add reaction mix and incubate for 60 min min
- Analyze with fluorescence microplate reader (Ex/Em = 535/587 nm) in end point mode
Other Notes
This product was previously called K589 Biovision Glycine Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
Glycine (GLY, G) is one of the 20 standard amino acids commonly found in proteins. Glycine's side chain is a hydrogen substituent, which makes it the smallest proteogenic and the only non-chiral amino acid. Basic functions of Glycine include the participation in the synthesis of creatine, glutathione, heme groups, and conjugated bile acids (bile salts). It is also present as one of the most abundant residues in the triple-helical structure of collagen.
Glycine acts as a glucogenic amino acid by regulating sugar levels in blood. Therefore, glycine supplementation has been used in patients suffering anemia, hypoglycemia and chronic fatigue. Glycine possesses both inhibitory and excitatory neurotransmitter functions in the brain stem and spinal cord. In cancer cells, glycine consumption is highly correlated to cancer cell proliferation via purine synthesis. Glycine uptake in cancer cell studies supports the role of this amino acid in tumorigenesis and malignancy.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycine a simple amino acid plays a critical role in various biological processes. It is often referred to as Gly or by its chemical formula C2H5NO2. Glycine has a molecular mass of approximately 75.07 g/mol. It is ubiquitously expressed in many tissues but is especially abundant in the central nervous system and connective tissues. Glycine acts as a building block for proteins and serves as an essential neurotransmitter in the central nervous system.
Biological function summary
Glycine influences the function of neurotransmitter systems. It serves as an inhibitory neurotransmitter in the spinal cord brainstem and retina through glycine receptors. Glycine forms part of collagen complexes in connective tissue contributing to structure and stability. Additionally glycine participates in the synthesis of other biomolecules like purines which are necessary for nucleic acid structure. Glycine's low molecular weight and small size enable it to fit into spaces within protein structures enhancing flexibility and function.
Pathways
Glycine influences the central nervous system's glycinergic pathways and is integral to the biosynthesis of serine and the glutathione pathway. Glycine's involvement in the glycinergic pathway associates it with glycine receptors and transporters that modulate synaptic transmissions. Within the glutathione pathway glycine works alongside glutamate and cysteine to form glutathione a vital antioxidant. This action highlights its cooperative role with proteins like the glutamate-cysteine ligase in cellular oxidative stress defense.
Glycine shows relevance to conditions such as glycine encephalopathy and schizophrenia. Excess glycine accumulation due to enzyme defects can lead to glycine encephalopathy impacting the central nervous system. Glycine concentrations and related receptor functions may also affect schizophrenia where neurotransmitter systems are disrupted. Glycine's involvement with NMDA receptors has been studied in these disorders suggesting its potential as a target for treatment strategies.
