Description
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.55 pg/mL,
Range:1.56 - 100 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human CCL18 ELISA Kit (ab211649) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL18 protein in cell culture supernatant, plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL18 with 0.55 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human CCL18 (macrophage inflammatory protein-4) belongs to the C-C motif containing chemokine family and contains a 20 amino acid (aa) signal peptide and a 69 aa residue mature protein. CCL18 was identified as a chemotactic factor for naïve T-cells, CD4+ and CD8+ T-cells, and nonactivated lymphocytes, suggesting that it plays an important role in immune responses. Additionally, MIP4 is mainly induced by Th2 type cytokines, such as IL-4 and IL-13, and inhibited by IFN-γ. High expression of MIP4 is seen in lung, lymph nodes, placenta, and macrophages derived from peripheral blood monocytes. Additionally, MIP4 is found in high levels in serum, plasma, and synovial fluid and in low levels in urine. Currently, a rodent homolog has not been identified.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CCL18 also known as pulmonary and activation-regulated chemokine (PARC) is a chemokine protein with a molecular weight of about 8.8 kDa. It is mainly expressed in the lung but also found in lymph nodes and placenta. CCL18 is secreted by activated macrophages and dendritic cells. As a chemokine it plays a role in immune system signaling where it attracts immune cells like T lymphocytes. Researchers utilize CCL18 ELISA kits to accurately measure its levels in biological samples providing insight into its functions.
Biological function summary
CCL18 plays an important role in modulating immune responses and recruiting immune cells to sites of inflammation. It is not part of a larger complex but works closely with other components of the immune system. CCL18 influences the activity of monocytes and the differentiation of T cells which are essential for mounting effective defense against pathogens. In inflammatory conditions increased CCL18 levels suggest its importance in controlling the magnitude and duration of immune responses.
Pathways
CCL18 is involved in the immune response and inflammatory pathways. It works particularly in chemokine signaling pathways and shares functional relations with proteins like CCR3 and CCR6 which act as receptors for various chemokines. The binding of CCL18 to these receptors on targeted cells facilitates the chemotaxis of immune cells steering the immune response to appropriate areas in the body where they are needed.
CCL18 has known associations with pulmonary fibrosis and various cancers. In pulmonary fibrosis CCL18 levels correlate with disease severity and progression indicating its potential role as a biomarker. The protein interacts with other signaling molecules including collagen and matrix metalloproteinases driving the fibrotic process. In cancers especially breast cancer high CCL18 expression often corresponds to tumor progression and metastasis. These interactions highlight its relevance not only in disease progression but also as a target for therapeutic interventions.
