Description
Key facts
Target:XRCC6See target data
Product details:
ab213658 is a Non Homologous End Joining Panel designed for the detection and characterization of key proteins involved in DNA double strand break (DSB) repair by non-homologous end joining (NHEJ). Histone H2A.X is phosphorylated on S139, termed gamma H2A.X, at sites of DNA damage. DNA DSB repair by NHEJ requires Ku70/Ku80 heterodimer binding to the DSB, as well as DNA Ligase IV for ligation of DNA ends.
This panel contains 5 recombinant rabbit monoclonal antibodies. They are provided as a sampler panel to allow you to easily evaluate each in your applications.
The panel contains:
- Rabbit monoclonal anti-Histone H2A.X antibody [EPR895] - ChIP Grade (ab124781)
- Rabbit monoclonal anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade (ab81299)
- Rabbit monoclonal anti-DNA Ligase IV/LIG4 antibody [EPR16531] (ab193353)
- Rabbit monoclonal anti-Ku70 antibody [EPR4027] (ab92450)
- Rabbit monoclonal anti-Ku80 antibody [EPR3468] (ab80592)
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ku70 and Ku80 together with DNA Ligase IV (LIG4) and Histone H2A.X play significant roles in maintaining genomic stability especially through the non-homologous end joining pathway. Ku70 also known as XRCC6 is a 70 kDa protein and Ku80 or XRCC5 is approximately 80 kDa. Both proteins are ubiquitously expressed meaning they are found in various cell types. These proteins form a heterodimer complex with DNA Ligase IV that recognizes and aligns broken DNA ends facilitating repair. Histone H2A.X variants including gamma H2A.X become phosphorylated upon DNA damage and serve as a signal for recruiting repair machinery to the double-strand breaks.
Biological function summary
Ku70 and Ku80 act as a DNA-binding complex that is important in double-strand break repair via the non-homologous end joining mechanism. The Ku heterodimer associates with DNA Ligase IV in a multiprotein complex to bridge and ligate broken DNA strands. Histone H2A.X including its phosphorylated form gamma H2A.X marks sites of DNA damage. The H2A.X variants contribute to chromatin remodeling making the DNA accessible to repair proteins and maintaining genomic integrity.
Pathways
Non-homologous end joining is one critical pathway where Ku70 and Ku80 are involved. This pathway operates independently of a homologous template and is essential for repairing DNA double-strand breaks. Another related pathway is the cell cycle checkpoint signaling where gamma H2A.X's phosphorylation helps signal and stall the cell cycle allowing time for repair. In these pathways Ku proteins and DNA Ligase IV work alongside proteins such as DNA-PKcs and XRCC4 to ensure effective DNA repair.
Disruptions in the non-homologous end joining pathway connect to cancer development and immunodeficiencies. Defects in Ku70 Ku80 or DNA Ligase IV function can lead to genomic instability contributing to the onset of various cancers. Mutations in these proteins also relate to conditions like severe combined immunodeficiency (SCID) as ineffective DNA repair impairs lymphocyte development. These connections highlight the critical roles these proteins play in disease prevention.
