Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:312 - 20000 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
The Human CD127 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213799) is designed for the quantitative detection of Human CD127 in cell culture supernatants and serum.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for CD127 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E21-I262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD127 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD127 amount of sample captured in plate.
CD127 (also known as interleukin-7 receptor or IL-7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukin 7 (IL-7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL-2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukin 2, 4, 7, 9, and 15. CD127 has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D) J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, CD127 antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD127 also known as IL-7R alpha or IL-7Rα is a protein found on the surface of cells involved in key immune functions. The CD127 protein has an approximate mass of 55-60 kDa. It is a component of the interleukin-7 receptor which also includes the common gamma chain. You can find CD127 expressed on various immune cells like T cells B cells and dendritic cells. As a marker CD127 is an important indicator of T cell maturity and function. Researchers often target CD127 in studies using antibodies in assay formats like ELISA to measure its expression level and function.
Biological function summary
CD127 plays an important role in regulating lymphocyte development and survival. It is part of the IL-7R complex partnering with IL-7 for signal transduction that promotes cell proliferation and survival. This interaction is essential for the development and homeostasis of T cells and B cells. In the absence of proper CD127 signaling lymphocytes may suffer from poor survival impacting overall immune competence. The IL-7-CD127 interaction informs researchers about mechanisms of immune modulation and resilience.
Pathways
CD127 participates in the JAK/STAT signaling pathway which is pivotal for transmitting information from extracellular signals to the cell nucleus influencing gene expression. This pathway plays a vital role in immune cell response. When IL-7 binds to the IL-7R complex on CD127 it initiates a cascade that involves proteins like JAK1 and STAT5. These proteins mediate responses necessary for cell proliferation and differentiation within immune system networks.
CD127 has implications in conditions like severe combined immunodeficiency (SCID) and autoimmune diseases. In SCID mutations or defects in CD127 cause impaired T cell development leading to compromised immune function. Additionally CD127 dysregulation can contribute to autoimmune disorders by affecting T regulatory cells creating an imbalance in immune response. Ocrevus a monoclonal antibody therapy used for multiple sclerosis indirectly relates to CD127 by targeting the B cells which rely on IL-7R signaling mechanisms for survival and function. Researchers continue to investigate CD127 to better understand its role in immune-related diseases.
