Description
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:78 - 5000 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A goat polyclonal antibody specific for BAFF-R has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S7-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.
BAFF-R (also known as Tumor necrosis factor receptor superfamily member 13C or B-cell-activating factor receptor) is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival
in vitro
and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BAFF-R also known as B-cell activating factor receptor or BAFF receptor plays a critical role in B-cell development and survival. BAFF-R is a receptor for the BAFF protein also referred to as B lymphocyte stimulator (BLyS). BAFF-R has a molecular mass of approximately 19 kDa. The expression of BAFF-R is found on B cells with higher presence on mature B cells compared to immature ones. The receptor is recognized as the main signaling partner for BAFF which forms the basis for many of its immune functions.
Biological function summary
BAFF-R is essential for the maturation and maintenance of peripheral B cells. The receptor contributes to the regulation of immune responses by ensuring B-cell homeostasis. It does not generally form part of a larger protein complex but instead functions as a membrane-bound receptor that transmits signals upon binding with the BAFF ligand. This signaling cascade supports the survival and proliferation of B cells which is fundamental for the adaptive immune system.
Pathways
BAFF-R is deeply involved in the NF-κB signaling pathway which is important for immune regulation and cell survival. Upon binding with BAFF BAFF-R recruits associated proteins to initiate the pathway and as a result promotes gene transcription important for B-cell function. Another connected pathway involves AKT a protein kinase that regulates B-cell metabolism and differentiation. Both NF-κB and AKT pathways help facilitate the immune functions tied to B cells directly through BAFF-R signaling.
BAFF-R demonstrates a notable link with autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. Overexpression of BAFF impacting interaction with BAFF-R contributes to such conditions by promoting the survival of autoreactive B cells. In SLE BAFF works with another receptor TACI influencing the proliferation of autoreactive cells. The persistent activation and dysfunction of the immune response through BAFF-R interactions exacerbate these autoimmune conditions.
