Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based (quantitative),
Assay Platform:Flow cytometer
Product details:
Annexin V- DY-634 PI Apoptosis Staining / Detection Kit (ab214484) contains Annexin V labeled with DY-634, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V – DY634 (red fluorescence) and the non-vital dye propidium iodide (PI) (orange fluorescence) allows the discrimination of intact cells (AnnexinV-DY-634 negative, PI Staining Solution negative), early apoptotic (Annexin V-DY-634 positive, PI Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-DY-634 positive, PI Staining Solution positive).
DY-634 can be used as direct replacement for APC.
For more apoptosis assays, review the full set of
Annexin V assays
, or the
apoptosis assay and apoptosis marker guide
Apoptosis is a regulated process of cell death that occurs during embryonic development as well as maintenance of tissue homeostasis. Inappropriately regulated apoptosis is implicated in different disease states, such as neurodegeneration disease and cancer. The apoptosis program is characterized by morphologic features, including loss of plasma membrane asymmetry and attachment, condensation off the cytoplasm and nucleus, and compaction and fragmentation of the nuclear chromatin. Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The human vascular anticoagulant, annexin V, is a 35-36 kDa Ca
dependent phospholipid binding protein that has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
