Description
Key facts
Detection method:Colorimetric,
Reacts with:Human,
Assay type:ELISA set,
Sensitivity:= 46.8 pg/mL,
Range:250 - 16000 pg/mL,
Assay Platform:Reagents
Product details:
Human IRAK4 Matched Antibody Pair Kit is an ELISA development kit to quantify Human IRAK4 by sandwich ELISA method with a sensitivity of 46.8 pg/mL.
How the Assay Works
A capture antibody specific of the antigen to be measured is coated on a 96-well plate. Samples are added to the wells followed by a biotin-labelled antibody. After elimination of the unbound antigen and detection antibody, a strepatavidin-HRP solution is added to the wells, followed by a TMB substrate. HRP catalyzes the oxidation of TMB in the presence of hydrogen peroxide, producing a blue-colored product. After the reaction is stopped, the solution turns yellow. The intensity of the yellow color, read at 450nm is directly proportional to the amount of antigen present in the sample.
Assay specificity
The Matched Antibody Pair Kit can be used to quantify native and recombinant human IRAK4.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
protocol summary
1. Coat the 96-well plate with the capture antibody overnight at 4C or 2 hours at room temperature on a plate shaker
2. Wash and block to reduce non specific binfing.
3. Add standards and samples to appropiate wells and incubate at room temperature.
4. Wash
5. Add the biotin-labelled detector antibody to all wells and incubate at room temperature
6. Wash
7. Add the HRP-Streptavidin solution and incubate at room temperature
8. Wash
9. Add the TMB substrate solution and incubate
10. Add stop solution and read at 450 nm
Complete your experiment with:
Matched Antibody Pair Kit ELISA Accessory Pack (10 x 96-well plates) (ab210905): contains 10 96-well plates, coating and blocking buffers, wash buffer, TMB substrate, stop solution and streptavidin-HRP solution.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IRAK4 also known as Interleukin-1 receptor-associated kinase 4 is an important protein with an approximate molecular mass of 51 kDa. This protein is a significant part of the signaling cascade initiated by the Interleukin-1 receptor (IL-1R) and Toll-like receptors (TLRs). Expressed in a variety of tissues including the spleen thymus and lung IRAK4 is involved in innate immune responses. The presence of IRAK4 protein facilitates the downstream signaling pathways that activate the transcription factors responsible for inflammatory cytokine production.
Biological function summary
IRAK4 plays an important role in mediating signaling in the innate immune system by forming complexes with other proteins such as IRAK-1. These complexes propagate signals that result in the activation of transcription factors like NF-kB and AP-1 which regulate the expression of inflammatory genes. Through these mechanisms IRAK4 influences the body's ability to respond to infections and stresses. Its critical involvement in these signaling pathways highlights its importance in maintaining immune homeostasis.
Pathways
IRAK4 is a pivotal player in the Toll-like receptor and IL-1 receptor signaling pathways which are central to the host defense against pathogens. It functions upstream activating the MyD88-dependent pathway resulting in the recruitment and phosphorylation of other kinases such as IRAK-1 and TRAF6. These interactions trigger the NF-kB signaling cascade thereby enhancing pro-inflammatory cytokine production that is essential for initiating immune responses.
Mutations or dysregulation of IRAK4 are linked to increased susceptibility to recurrent bacterial infections and sepsis. Patients with IRAK4 deficiency have impaired NF-kB activation leading to a weakened inflammatory response. Additionally overexpression of IRAK4 has been observed in certain autoimmune diseases such as rheumatoid arthritis where the persistent activation of TLR signaling contributes to chronic inflammation. The role of IRAK4 in these disorders ties closely with its interaction with proteins like TRAF6 influencing disease development and progression.
