Description
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Assay type:Enzyme activity (quantitative),
Assay time:20m,
Assay Platform:Microplate reader
Product details:
Glucose Oxidase Activity Assay Kit ab219924 uses a simple colorimetric assay to measure glucose oxidase (GOx) activity in cell and tissue extracts, and biological fluids. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.
The glucose oxidase assay protocol uses a red probe to monitor glucose oxidase activity, leading to an increase in absorbance that can be easily detected at OD575 nm.
Glucose oxidase assay protocol summary:
- add samples and standards to wells
- add glucose oxidase reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 10-30 mins at 37°C
Glucose oxidase (notatin, GOx, EC 1.1.3.4) is a dimeric enzyme that catalyzes oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glucose oxidase also known as GOX is an enzyme that catalyzes the oxidation of beta-D-glucose to glucono delta-lactone releasing hydrogen peroxide as a by-product. This enzyme with a molecular weight of approximately 160 kDa is commonly found in fungi like Aspergillus and Penicillium species. Glucose oxidase is predominantly expressed in the extracellular space of these organisms facilitating its role in glucose metabolism and defense mechanisms. It plays an important role in various biochemical assays including the 'glucose oxidase assay' 'glucose oxidase test' and 'glucose oxidase kit' often used for glucose quantification in diverse samples.
Biological function summary
Glucose oxidase is significant in both metabolic pathways and environmental defense. The enzyme acts as a part of a larger enzymatic reaction complex that includes peroxidase which together help in the glucose oxidase reaction to maintain cellular redox balance. Additionally the production of hydrogen peroxide by glucose oxidase serves as a chemical defense mechanism against microbial infections. This property is harnessed in technical applications making glucose oxidase a valuable tool in biotechnology for both its metabolic impacts and antimicrobial properties.
Pathways
Glucose oxidase is an important enzyme in the carbohydrate metabolism and oxidative stress response pathways. It acts by converting glucose into glucono delta-lactone which can then enter further metabolic processes. The enzyme interacts closely with other proteins particularly those involved in oxidative phosphorylation such as catalase which neutralizes the hydrogen peroxide generated during the glucose oxidase reaction. These interactions help modulate cellular energy production and maintain oxidative homeostasis highlighting its role within broader metabolic networks.
Glucose oxidase activity is relevant to conditions such as diabetes and neurodegenerative diseases. The enzyme's function in glucose monitoring tools plays a critical role in diabetes management where accurate glucose measurement is important. Additionally altered levels of hydrogen peroxide due to dysregulated glucose oxidase activity is implicated in oxidative stress which can contribute to neurodegenerative disorders like Alzheimer's disease. In these contexts the enzyme's interaction with proteins like catalase and superoxide dismutase becomes significant as these proteins aid in mitigating oxidative damage which is central to the pathology of such diseases.
