Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based,
Assay Platform:Microplate reader, Fluor. microscope, Flow cyt.
Product details:
Caspase 6 (active) Staining Kit - Green Fluorescence (ab219936) is a sensitive fluorometric assay to measure caspase 6 activation in live cells. The assay uses FAM-VEID-FMK, which binds irreversibly to active caspase 6 in apoptotic cells. The fluorescent intensity of the FAM-VEID-FMK signal is proportional to the amount of active caspase 6 and can be easily detected at Ex/Em = 490/525 nm by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.
Caspase activity assay kits are based on fluorescent inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Overexpression of caspase 6 promotes programmed cell death. It has been proven that caspase 6 has substrate selectivity for the peptide sequence Val-Glu-Ile-Asp (VEID). This kit uses FAM-VEID-FMK as a fluorescent indicator for caspase 6 activity. FAM-VEID-FMK irreversibly binds to activated caspase 6 in apoptotic cells.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
