Description
Key facts
Detection method:Fluorescent,
Sample types:Tissue Lysate, Urine, Plasma, Serum, Other biological fluids, Cell Lysate,
Assay type:Quantitative,
Sensitivity:= 40 nM,
Assay Platform:Microplate reader
Product details:
Total Choline Detection Kit (ab219944) provides a sensitive fluorescence-based assay for quantifying choline in cell and tissue lysates and biological fluids. The amount of choline is proportional to the concentration of hydrogen peroxide formed in the choline oxidase-mediated enzyme coupling reaction cycle, which in turn is detected with our proprietary Red Indicator. The increase in fluorescence can be easily detected at an optimal Ex/Em = 540/590 nm. The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.
The assay detects as little as 4 picomole choline in 100 uL assay volume (40 nM), making it one of the most sensitive assays available in the market.
This product was previously known as Total Choline Detection Kit.
Other Notes
Choline is an essential nutrient. Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (choline synthesis). It is a major source of methyl group via its metabolite, trimethylglycine that participates in the S-adenosylmethionine synthesis pathways. Choline deficiency may cause liver disease, atherosclerosis and possibly neurological disorders. Despite its importance in the central nervous system as a precursor for acetylcholine and membrane phosphatidylcholine, the role of choline in mental illness has been little studied.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Total choline is a collection of water-soluble quaternary ammonium compounds that includes phosphocholine glycerophosphocholine and free choline. It plays a mechanical role in cell membrane synthesis and signaling. Alternate names for choline include trimethylammonioethanol and vitamin B4. Choline weighs about 104.17 daltons in its free form. It is primarily expressed in the liver but also found in the brain and other organs. Commercial kits such as choline assay kits and choline test kits often assess choline concentrations using enzymes like choline oxidase.
Biological function summary
Choline facilitates several critical cellular functions including neurotransmitter synthesis where it is a precursor to the neurotransmitter acetylcholine. Choline does not act alone; it functions as part of complexes with phospholipids for efficient cell membrane integrity and fluidity. Choline's presence in cell membranes supports structure and aids in signal transmission and lipid transport.
Pathways
Choline occupies an essential position in the methylation and phospholipid synthesis pathways. These pathways involve enzymes such as choline kinase and phosphatidylethanolamine N-methyltransferase. Choline works closely with amino acids like methionine in methyl metabolism and it interacts with proteins like SAM (S-adenosylmethionine) in these biochemical processes impacting DNA synthesis and repair through its role as a methyl-group donor.
Choline deficiency has links to non-alcoholic fatty liver disease and neural tube defects. Choline's connection to liver metabolism involves protein interactions with enzymes like fatty acid synthase highlighting the impact of imbalanced choline levels on lipid metabolism. In neurological disorders like Alzheimer's disease choline deficiency impacts acetylcholine levels revealing its important connections to cognitive function alongside interactions with beta-amyloid proteins.
