Description
Key facts
Assay time:1h,
Sample types:Tissue, Suspension cells, Adherent cells
Product details:
ab221031 Histone Extraction Kit - Rapid / Ultra-pure enables the extraction of core histones from mammalian cells or tissues in 1 hour using a simple procedure with 20 minutes hands-on time. As post-translational modifications, such as acetylation, methylation and phosphorylation are maintained, histones extracted using this kit can be used in a wide variety of downstream applications.
This kit is compatible with three distinct protocols: Standard, Rapid and Ultra-pure Histone Extraction. The
Standard Extraction
protocol is a 1 hour procedure (of which 20 minutes is hands-on) that is suitable for histone extraction from a wide range of cell lines and tissues. The
Rapid Extraction
protocol can be used to prepare a crude histone extract quickly for use in applications where histone purity is not critical. It is also recommended for histone extraction using fragile cell lines or in instances where extraction of cytoplasmic histones is desirable. This protocol takes 45 minutes and requires 15 minutes of hands-on time. The
Ultra-pure Histone Extraction
protocol is a 75-minute procedure (with 25 minutes hands-on time) that yields very pure histones from a wide range of cell lines and tissues.
Each kit contains reagents to perform 100 extractions, where each extraction requires 2 x 10
cells or 20 mg of tissue starting material. Typical yields are around 80 μg of histones from 2 x 10
HeLa cells.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols
