Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum, Tissue, Cell culture supernatant, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 21 pg/mL,
Range:0.031 - 2 ng/mL,
Assay time:4h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
The Human CA2 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222881) is designed for detection of human CA2 in plasma, serum, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CA2 in approximately 4 hours. A polyclonal antibody specific for human CA2 has been pre-coated onto a 96-well microplate with removable strips. CA2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CA2, which is recognized by a Streptavidin-Peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Carbonic anhydrase 2 (CA2, CAC), also known as carbonate dehydratase II and carbonic anhydrase II, belongs to the alpha-carbonic anhydrase family. It contains 259 amino acid residues and has a molecular mass of 29 kDa. CA2 catalyzes reversible hydration of carbon dioxide and is expressed at high levels in osteoclasts during bone resorption. CA2 supports the transport of bicarbonate ions, sodium ions, and water from the blood to the cerebrospinal fluid.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
