Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 68.2 pg/mL,
Range:195.3 - 12500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Cardiac Troponin T ELISA Kit ab223860 is a rapid single-wash 90-min Sandwich ELISA to measure Human Cardiac Troponin T in cell culture media, serum. This SimpleStep sensitivity is 68.2 pg/mL.
How the assay works
Human Cardiac Troponin T SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Cardiac Troponin T ELISA Kit ab223860 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Cardiac Troponin T antibody [EPR20266-143] - BSA and Azide free (Capture)
ab252655
- Anti-Cardiac Troponin T antibody [EPR20266-143-6] - BSA and Azide free (Detector)
ab252655
Cardiac Troponin T is one of the three tropomyosin binding subunit that makes up the troponin complex with TnI and TnC being the other two subunits. The troponin complex is essential to the contraction of skeletal and heart muscles. Troponin T specifically binds to tropomyosin and helps position it on actin. Studies have shown elevated levels of troponin T after myocardial infarction and significantly associated with the incidence of heart failure and cardiovascular death.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
