Description
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P69291,
Animal free:No,
Carrier free:No,
Species:Influenza A virus (A/Beijing/353/1989(H3N2)),
Storage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, -80°C, Appropriate long-term storage conditions--20°C, -80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Influenza A Virus Nucleoprotein also known as NP or nucleoprotein influenza A plays a significant mechanical role in the virus life cycle. It is an essential structural component that encapsidates viral RNA forming ribonucleoprotein complexes important for replication and transcription. Influenza A nucleoprotein has a molecular weight of approximately 56 kDa and is expressed in infected host cells. Within the influenza virion the nucleoprotein interacts with the viral RNA and polymerase complex ensuring the integrity and functionality of the viral genome.
Biological function summary
The influenza A nucleoprotein is involved in regulating viral RNA synthesis and acts as a platform for the assembly of viral components. It does not directly form stable complexes with host proteins but it is central to the ribonucleoprotein complexes that are vital for the virus life cycle. This nucleoprotein assists in the virus's ability to hijack the host cellular machinery favoring its replication and infection processes. While the nucleoprotein itself does not form direct complexes with host proteins its interactions are significant for facilitating various stages of the viral life cycle.
Pathways
The influenza A nucleoprotein operates within the viral replication and transcription pathways. It associates with proteins such as PB1 PB2 and PA of the polymerase complex which is important for viral RNA synthesis. The nucleoprotein ensures the proper packaging of the viral genome to be recognized by the polymerase complex playing a pivotal role in the viral replication process. Through these pathways it indirectly impacts the host immune response as the virus must evade host defenses to ensure replication success.
The nucleoprotein influenza A is primarily linked to influenza infections a major respiratory illness. Influenza can manifest in a mild to severe form occasionally leading to pandemics. The nucleoprotein is an attractive target for antiviral drug development given its essential role in the viral life cycle. Moreover studies have shown that variations and mutations in the nucleoprotein can affect viral virulence and the outcome of infection. By understanding its mechanisms and interactions with other viral proteins like HA and NA researchers can better develop strategies to combat influenza outbreaks.
