Description
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 10.9 pg/mL,
Range:31.25 - 2000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human C1 inhibitor ELISA Kit ab224883 is a rapid single-wash 90-min Sandwich ELISA to measure Human C1 inhibitor in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 10.9 pg/mL.
How the assay works
Human C1 inhibitor SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human C1 inhibitor ELISA Kit ab224883 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-C1 inhibitor antibody [EPR20165-39] - BSA and Azide free (Capture)
ab244940
- Anti-C1 inhibitor antibody [EPR20165-84] - BSA and Azide free (Detector)
ab244940
C1 inhibitor, also known as C1 esterase inhibitor and Serpin G1, is a protease inhibitor belonging to the serpin superfamily, and is encoded by the gene SERPING1. C1 inhibitor is a 478 -amino acid containing protein, and is heavily glycosylated with an approximate 105 kDa molecular mass. The primary function of C1 inhibitor is the inhibition of the classical complement cascade to suppress spontaneous activation by forming an inactive complex with C1r and C1s proteases. In addition, C1 inhibitor also inactivates proteases of the fibrinolytic, clotting, and kinin pathways. The levels of C1 inhibitor in blood fluctuate during inflammation, and deficiencies of C1 inhibitor are associated with hereditary angioedema.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C1 inhibitor also known as C1 esterase inhibitor or C1-INH is a serpin family protein that plays a significant role in inhibiting the complement system. This protein found in human serum has a molecular mass of about 104 kDa. C1 inhibitor is predominantly expressed in the liver and secreted into the bloodstream where it functions to regulate several proteolytic cascades. As a glycoprotein it also contains significant carbohydrate moieties that aid in its stability and function.
Biological function summary
As an inhibitor of the classical pathway of the complement system C1 inhibitor controls the activation of C1 esterase. This interaction prevents the uncontrolled activation of downstream components of the complement cascade which could result in tissue damage. C1 inhibitor also participates in regulating the kallikrein-kinin system preventing excessive inflammation. It achieves these roles not alone but as a part of complexes that interact with other proteins to maintain homeostasis within the immune system.
Pathways
The role of C1 inhibitor intersects with critical inflammatory modulatory pathways such as the complement pathway and the kallikrein-kinin system. These pathways are essential for proper immune response modulation preventing excessive inflammation and maintaining vascular permeability. C1-INH acts alongside proteases like kallikrein and proteins like C4 and C2 within these pathways to regulate the initiation and progression of the immune response during infections or insults.
Inadequate levels or functional defects in C1 inhibitor lead to hereditary angioedema a condition characterized by recurrent episodes of severe swelling. This disease highlights the importance of C1-INH in controlling vascular leakage and inflammation. Moreover C1 inhibitor has been implicated in autoimmune diseases like systemic lupus erythematosus where its regulatory kinetics with proteins such as C1q play a part in managing excessive complement activation. These associations highlight the important protective role of C1 inhibitor in health and disease.
