Key facts
Target:ITGAMSee target data
Product details:
Microglia Marker (CD11b, CD45, Iba1, TMEM119) Antibody Sampler Panel (ab226482) contains antibodies against the key microglia markers, CD11b, CD45, Iba1, and TMEM119.
Microglia are the macrophages of the brain and spinal cord and act as an immune defense in the central nervous system (CNS).
The rabbit monoclonal antibodies in this panel were selected for their exceptional performance in IHC, alongside other applications. Please see the individual datasheets for additional information.
Please note that Anti-TMEM119 antibody [28-3] is for mouse reactivity only.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email
[email protected].
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD11b CD45 Iba1 and TMEM119 are markers commonly associated with microglia the resident immune cells of the central nervous system (CNS). CD11b is part of a larger integrin complex also known as integrin alpha M and has a mass of approximately 165 kDa. It is predominantly expressed on the surface of myeloid cells encompassing not just microglia but also macrophages and neutrophils. CD45 a tyrosine phosphatase exists in multiple isoforms with expression found broadly across hematopoietic cells including microglia. Iba1 or allograft inflammatory factor 1 is a smaller cytoplasmic protein of about 147 amino acids vital for microglial activation and is commonly used in Iba1 staining for identifying these cells in immunohistochemistry (IHC). TMEM119 serves as a specific marker for microglia in the CNS distinguishing them from other macrophage populations.
Biological function summary
These markers play significant roles in the immune functions of microglia. CD11b facilitates cell adhesion and migration through its role in the leukocyte adhesion cascade an essential part of immune response. CD45 participates in regulating signal transduction being critical in T and B cell receptor signaling. Iba1 is involved in the microglia's response to injuries contributing to phagocytosis and membrane ruffling. TMEM119 helps identify microglia in mature CNS offering insights into microglial physiology and pathology without interfering in signaling pathways since it is a surface marker with an unknown specific role.
Pathways
CD11b and CD45 interact within the immune system's integrin and leukocyte activation pathways. CD11b pairs with CD18 to form the Mac-1 receptor integral to the leukocyte adhesion and migration process. CD45 modulates T cell and B cell receptor signaling pathways through dephosphorylation of specific Src family kinases. These pathways contribute to the modulation of immune functions particularly in inflammatory responses in the CNS. Iba1 correlates functionally less with specific pathways but indicates active microglial participation in neuroinflammatory responses.
These markers are critical in studying neurodegenerative diseases such as Alzheimer’s disease and multiple sclerosis which involve significant microglial activation. Iba1 and TMEM119 being microglia-specific aid in understanding the contribution of these immune cells to neurodegeneration. CD11b's expression increases during Alzheimer’s disease associating it with amyloid plaque clearance. Meanwhile CD45 also links to Alzheimer’s as it modulates microglial activation and influences amyloid-beta clearance. These proteins collectively provide critical information for research targeting microglial function and modulation in CNS diseases.
