Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 11.7 pg/well,
Range:39.1 - 2500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human BAG3 ELISA Kit ab227900 is a rapid single-wash 90-min Sandwich ELISA to measure Human BAG3 in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 11.7 pg/weLL.
How the assay works
Human BAG3 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human BAG3 ELISA Kit ab227900 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Bag3 antibody [EPR20207-4] - BSA and Azide free (Capture)
ab244958
- Anti-Bag3 antibody [EPR20207-109] - BSA and Azide free (Detector)
ab244958
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Bcl-2-associated athanogene 3 commonly known as Bag3 is a protein with a molecular weight of approximately 74 kDa. It plays an important role in apoptosis regulation and stress responses by interacting with heat shock proteins. Bag3 is expressed in various tissues including the heart skeletal muscles and certain types of neurons. It functions mechanically by binding to chaperones like Hsp70 modulating their activity and helping in protein folding and degradation processes.
Biological function summary
Bag3 contributes to cell survival by assisting in protein homeostasis and mitigating stress-related cellular damage. It acts as a co-chaperone and forms part of complexes with Hsp70 HspB8 and CHIP among others. These interactions enhance the cellular capacity to handle misfolded proteins preventing the aggregation that could lead to cell toxicity. In muscle cells Bag3 plays a role in maintaining structural integrity by participating in the assembly of myofibrils critical for muscle function.
Pathways
The protein is involved in autophagy and apoptosis regulation. It actively participates in the ubiquitin-proteasome system and chaperone-mediated autophagy collaborating closely with proteins like HspB8 and Hsp70. The integration of Bag3 within these pathways highlights its involvement in removing damaged proteins and preserving cellular function during stress conditions. Additionally Bag3 contributes to the regulation of the NF-kB pathway which is involved in the immune response and inflammation.
Mutations or dysregulation of Bag3 associate with conditions such as myopathy and certain cancers. In myopathy aberrations in Bag3 affect muscle resilience and repair leading to structural muscle defects. In cancer Bag3's role in inhibiting apoptosis promotes tumor cell survival making it a target for therapeutic interventions. The protein has connections with p53 in cancer contexts where it influences cell death and survival decisions. Understanding Bag3's interactions and functions in these diseases opens new opportunities for developing treatments targeting these pathways.
