Description
Key facts
Detection method:Colorimetric,
Sample types:Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 30 pg/mL,
Range:312.5 - 20000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Albumin ELISA Kit ab227933 is a rapid single-wash 90-min ELISA kit to measure Human Albumin in urine, serum and plasma. This SimpleStep ELISA® sensitivity is 30 pg/mL.
How the assay works
Human Albumin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and purified Human IgA protein in serum, plasma, milk, urine, saliva, and cell culture media samples only. Cell and tissue extracts have not been tested with this kit.
Human Albumin ELISA Kit ab227933 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
Save your precious samples
Our 384-well Human Albumin ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
ab244051
ab244696
ab244962
Related and recommended products
Human Albumin ELISA kit ab227933 is commonly used by researchers to measure liver or kidney function.
Liver function ELISA assays include Human ALT ELISA kit
ab234578
and Human AST ELISA Kit
ab263881
These ELISA kits are also available for Mouse/Rat:
- Mouse Albumin ELISA kit
ab207620
- Mouse ALT ELISA kit
ab282882
- Mouse AST ELISA kit
ab263882
- Rat Albumin ELISA kit
ab235642
- Rat ALT ELISA kit
ab234579
- Rat AST ELISA kit
ab263883
Kidney function marker assays include:
- Creatinine Assay Kit
ab65340
– suitable for use as a serum creatinine assay and a urine creatinine assay.
- Albumin (BCG) Assay Kit (Colorimetric)
ab235628
– suitable for use with serum
- Urea Assay Kit
ab83362
– suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
