Description
Key facts
Detection method:Luminescent,
Sample types:Cell culture extracts, Adherent cells,
Assay Platform:Microplate reader
Product details:
Luciferase Reporter Assay Substrate Kit - Renilla (ab228546) provides a fast and sensitive method to detect the luciferase from sea pansy (Renilla reniformis). It uses a proprietary luminogenic formulation to quantify
Renilla luciferase
activity in cell-based assays. The formulation generates a luminescent product that gives strong luminescence upon interaction with Renilla luciferase. The kit provides all the essential components. It has high sensitivity and can be performed in a convenient 96-well and 384-well microtiter-plate format. The "glow-type" signal with a half-life of one hour provides a consistent signal across large number of assay plates. The assay is compatible with standard cell growth media. This kit enables the measurement of primary expression or gene expression with wild type and the synthetic hRluc genes.
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Renilla luciferase since these reporters are smaller and do not require the presence of ATP.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
