Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based (quantitative),
Assay Platform:Microplate reader
Product details:
Calcein AM Assay Kit (ab228556) can detect as low as 50 viable cells in less than 30 minutes.
How the Calcein AM assay works
Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. During the Calcein AM assay, hydrolysis of Calcein AM by intracellular esterases produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells.
The Calcein AM assay can be used for cell proliferation, cell viability, and cytotoxicity studies.
Calcein AM assay protocol summary
- prepare cells
- prepare Cell Dye II/Calcein AM solution
- add prepared Cell Dye II/Calcein AM solution to each well
- incubate at 37ºC for 30 minutes
- read fluorescence (Ex/Em = 485/530 nm)
Review the to learn about more kits to perform a , and .
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
