Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Homogenate,
Assay type:Enzyme activity (quantitative),
Sensitivity:< 0.1 mU/well,
Assay Platform:Microplate reader
Product details:
How the assay works
In Succinate Dehydrogenase Activity Assay Kit (Colorimetric), Succinate Dehydrogenase converts succinate to fumarate, and transfers the electron to an artificial electron acceptor (Probe), which changes the color from blue to a colorless product (depending upon the sample enzymatic activity).
Succinate Dehydrogenase Activity Assay Kit (Colorimetric) protocol summary:
- Rapidly homogenize sample tissue / cells in assay buffer
- Centrifuge sample and transfer supernatant to fresh tube
- Add sample to plate well
- Add reaction buffer and mix well
- Immediately measure absorbance (600 nm) in kinetic mode for 10 –30 minutes at 25°CThe kit provides enough reagents for 100 assays.
How other researchers are using
Succinate Dehydrogenase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
- Mouse liver mitochondria 1
- Rat brain tissues 2
- HepG2 and MCF-7 cells 3
References: 1-Rome F al. 2022
2 Almohaimeed H et al. 2022
3- Liu X et al. 2021
This product is manufactured by BioVision, an Abcam company and was previously called K660 Succinate Dehydrogenase Activity Colorimetric Assay Kit. K660-100 is the same size as the 100 test size of ab228560.
Succinate Dehydrogenase (SDH) (EC 1.3.5.1) or succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme complex, which is bound to the inner mitochondrial membrane. SDH participates in both the citric acid cycle and electron transport chain. In mammals and many bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone. SDH deficiency in humans leads to a variety of phenotypes including Leigh syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent studies show that SDH can prevent the generation of ROS (reactive oxygen species); therefore, measurement of succinate dehydrogenase activity has wide applications.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complex II also known as succinate dehydrogenase (SDH) or succinate-ubiquinone oxidoreductase plays an important mechanical role in the mitochondrial electron transport chain. It catalyzes the oxidation of succinate to fumarate while reducing ubiquinone to ubiquinol. The enzyme complex is approximately 140 kDa in mass and resides in the inner mitochondrial membrane. Complex II is expressed in most tissues particularly in high-energy demand tissues such as the heart and skeletal muscles.
Biological function summary
Complex II functions as part of the larger electron transport chain complex and plays a role in the Krebs cycle. It links two critical metabolic pathways converting succinate to fumarate while transferring electrons to the electron transport chain. This makes it integral for proper cellular respiration and energy production. The complex consists of multiple subunits and utilizes co-factors like FAD and iron-sulfur clusters for enzymatic activity. It is also a part of the supercomplexes that optimize the efficiency of oxidative phosphorylation.
Pathways
Complex II plays a significant role in both the citric acid cycle and oxidative phosphorylation. It acts as a connecting bridge between these two pathways facilitating the flow of electrons. Complex II works alongside other proteins such as complex I and complex III to maintain the electron transport chain's function and energy production. Succinate dehydrogenase transfers electrons within the chain directly affecting the generation of ATP by complex V (ATP synthase).
Dysfunction of complex II is associated with mitochondrial diseases and cancers. Mutations or deficiencies in its subunits can lead to conditions like Leigh syndrome and hereditary paraganglioma. These conditions frequently involve other mitochondrial proteins and complexes such as complex I which can exacerbate the electron transport chain dysfunction. In cancers alterations in succinate dehydrogenase activity can result in oncogenic metabolisms by falsely stabilizing hypoxia-inducible factors linking it further with the genetic and metabolic regulation.
