Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Suspension cells, Adherent cells,
Assay Platform:Microplate reader
Product details:
How the assay works
In this Lipid Peroxidation (MDA) Assay, the MDA Color Reagent reacts with MDA to generate a blue color product which is measured at 695 nm with absorbance microplate readers.
Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol. Malondialdehyde (MDA) is one of the most commonly used biomarkers for lipid peroxidation.
Running an MDA assay has historically relied on a reaction with thiobarbituric acid (the TBARS assay) to generate a product that can be measured colorimetrically at 532 nm or fluorimetrically at Ex/Em = 530/550 nm.
However, the TBARS assay has quite a few limitations:
- the reaction is not specific to MDA,
- the TBA-MDA reaction needs be run under acidic conditions,
- the TBARS assay needs be run under high temperature, commonly at 90-100 °C.
Lipid Peroxidation (MDA) Assay Kit (Colorimetric) protocol summary:
- add samples and standards to wells
- add MDA color reagent and incubate for 10-30 min at room temp
- add reaction solution and incubate for 30-60 min at room temp
- analyze with microplate reader
How other researchers are using
Lipid Peroxidation (MDA) Assay Kit (Colorimetric) has been used in a variety of sample type including:
- Human GC cell lines include SNU719, AGS, MKN45, and HGC-27 1
- Human breast cancer MDA-MB-468 2
- Mouse Kidney tissues 3
References:
1 - Wang J et al. 2024; 2 -Alsrhani A et al. 2023; 3 - Pham T et al. 2023.
Related and recommended products
See an alternative kit, TBARS assay kit for MDA measurement:
- Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
