Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Urine, Plasma, Serum, Cell Lysate,
Assay type:Quantitative,
Sensitivity:= 1 nmol/well,
Assay time:1h 30m,
Assay Platform:Microplate reader
Product details:
Griess Assay Kit / Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.
Griess assay principle
In the Griess reaction, Nitrite is reduced to nitrogen oxide using Griess Reagent I. Nitrogen oxide then reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at 540 nm.
Griess Reagent I is 1% Sulfanilamide in 5% Phosphoric Acid.
Griess Reagent II is 0.1% NED (N-(1-Naphthyl)ethylenediamine dihydrochloride) in water.
The two-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.
Griess assay protocol summary
- add samples and standards to wells
- sequentially add Griess Reagent I, Griess Reagent II, assay buffer to wells
- incubate for 10-min at room temperature
- analyse on a plate reader
Related Nitrite / Nitrate assays
- To measure total nitrate/nitrite levels, use Nitric Oxide Assay Kit
ab65328
Other Notes
This product was previously called K544 Biovision Nitrite Assay Kit (Griess Reagent). Abcam acquired Biovision in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols
