Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Plasma, Serum,
Assay type:Quantitative,
Assay Platform:Microplate reader
Product details:
Phospholipid Assay Kit (Colorimetric/Fluorometric) (ab234050) is a simple, accurate and reproducible way to measure total choline-containing phospholipids in biological samples such as serum and plasma.
This kit provides a high-throughput assay of inhibitors/inducers affecting phospholipid metabolism. The signal is directly proportional to the phospholipid concentration of phospholipids in the sample and can detect phospholipids in the range of 0.1 to 10 nmol per sample.
This product is manufactured by BioVision, an Abcam company and was previously called K351 Phospholipid Assay Kit (Colorimetric/Fluorometric). K351-100 is the same size as the 100 test size of ab234050.
Phospholipids are a major component of lipid bilayer membranes. In addition, phospholipids are biologically important molecules as they are closely involved in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline moiety. In human serum and plasma, almost 95% of the phospholipids are choline-containing phospholipids such as phosphatidylcholine and sphingomyelin. The remaining 5% is mainly choline-free phospholipids (i.e. phosphatidylethanolamine and phosphatidylserine). Phospholipid concentration is considered a disease biomarker as it can be altered in many lung, liver and heart diseases.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phospholipids are essential structural components of cellular membranes also known as glycerophospholipids. Their structure typically consists of a hydrophilic head and two hydrophobic tails composed of fatty acids. Phospholipids have a molecular mass range because of their varying fatty acid chains but generally fall around 750 Daltons. They are universally expressed in all cell types from bacteria to eukaryotes due to their fundamental role in membrane formation. Phospholipids also serve as substrates for several signaling molecules adding complexity to their function.
Biological function summary
Phospholipids are vital in maintaining the integrity and functionality of membranes. They form lipid bilayers providing impermeable barriers for cells and organelles. Phospholipid molecules participate in cell signaling serving as precursors for signaling lipids like phosphatidylinositol. These molecules can organize into complex structures known as liposomes or micelles depending on their environment. In addition they interact extensively within membrane proteins affecting the protein's structure and function.
Pathways
Phospholipids play a significant role in the signal transduction and apoptosis pathways. In the signal transduction pathway phosphatidylinositol derivatives act as secondary messengers regulating various cellular responses. Proteins such as phospholipase C and kinase enzymes interact closely with these derivatives illustrating their involvement in complex cellular processes. The apoptosis pathway also relies on phospholipids with the release of ceramide from sphingomyelin degradation triggering programmed cell death. These interactions highlight important aspects of cellular regulation and homeostasis.
Phospholipids are linked to cardiovascular diseases and metabolic disorders. Imbalances or mutations affecting phospholipid synthesis can lead to atherosclerosis where plaque formation in arteries occurs. Disorders such as Niemann-Pick disease result from dysfunctional phospholipid metabolism specifically involving the protein acid sphingomyelinase. Its dysfunction leads to abnormal lipid accumulation in tissues. The understanding and manipulation of phospholipid pathways offer potential therapeutic approaches for these diseases.
