Description
Key facts
Detection method:Fluorescent,
Sample types:Tissue Lysate, Cell Lysate,
Assay Platform:Microplate reader
Product details:
Chymotrypsin Assay Kit (Fluorometric) (ab234051) uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore. The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin.
This product is manufactured by BioVision, an Abcam company and was previously called K352 Chymotrypsin Activity Assay Kit (Fluorometric). K352-100 is the same size as the 100 test size of ab234051.
Chymotrypsin (EC 3.4.21.1) is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine).
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Chymotrypsin also known as chymotrypsinogen when in its inactive precursor form and chymotrypsine stands out as a serine protease enzyme with a molecular weight around 25 kDa. Mechanically chymotrypsin catalyzes the hydrolysis of peptide bonds especially those containing aromatic amino acids like phenylalanine tyrosine and tryptophan. The enzyme exhibits a preference for cleaving at the carboxyl side of these amino acids. Chymotrypsin is mainly expressed in the acinar cells of the pancreas where it aids in the digestive process. The enzyme becomes active upon cleavage by trypsin another serine protease forming chymotrypsin and trypsin as active proteolytic enzymes.
Biological function summary
The enzyme plays an essential role in the digestion of proteins within the small intestine. Chymotrypsin contributes significantly to breaking down dietary proteins into smaller peptides and amino acids which are easier to absorb. During this process chymotrypsin partners with other pancreatic enzymes such as trypsin making up a proteolytic cascade. Chymotrypsin activity ensures efficient protein digestion which is important for nutrient absorption and metabolism. The enzyme's product which results from substrate cleavage is integral to various physiological processes.
Pathways
Chymotrypsin is intricately involved in the digestive enzymatic cascade that ensures proper digestion and absorption of nutrients. The proteolytic activity of chymotrypsin and trypsin in the digestive system forms part of the broader system of exocrine pancreatic secretion. This pathway which involves various enzymes like elastase and chemotrypsin facilitates the efficient processing of proteins and is critical for maintaining metabolic homeostasis. Chymotrypsin's interaction with other enzymes highlights its significance in these pathways.
Abnormal chymotrypsin activity can link to certain pancreatic disorders such as chronic pancreatitis where enzyme secretion disrupts. This disruption may relate through the dysfunction of other enzymes including trypsin. Moreover hereditary pancreatitis associates with mutations affecting the regulation of the enzyme or its precursor chymotrypsinogen. Understanding these associations helps to elucidate the role of chymotrypsin protein in various pathological states shedding light on the importance of maintaining its normal function to prevent disease progression.
