Description
Key facts
Detection method:Fluorescent,
Sample types:Tissue Lysate, Tissue Homogenate, Cell Lysate,
Assay type:Quantitative,
Assay Platform:Microplate reader
Product details:
Collagenase (Collagen Degradation/Zymography) Assay Kit (Fluorometric) (ab234624) employs a highly quenched collagen substrate which upon cleavage by a suitable collagenase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of collagenase activity (as low as 0.6 mCDU for bacterial collagenase) in biological samples.
Other Notes
This product was previously called K490 Collagenase (Collagen Degradation/Zymography) Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
Collagenases, members of the matrix metalloproteinase (MMP) family, are endopeptidases that digest native collagen in the triple helix region and are involved in the physiological and pathological turnover of connective tissues. Collagens are the major fibrous component of animal extracellular connective tissue. Bacterial collagenases differ from vertebrate collagenases in that they exhibit broader substrate specificity. Collagenases may play a role in facilitating tumor cell invasion of the extracellular matrix at multiple stages of the metastatic process. Collagenases have also been approved for medical uses for the treatment of Dupuytren's contracture, Peyronie's disease and wound healing.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
