Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:= 0.2 mM,
Assay Platform:Microplate reader
Product details:
FRAP Assay Kit ab234626 provides a quick, sensitive and easy way for measuring the antioxidant capacity of various biological samples. FRAP assay stands for Ferric Reducing Antioxidant Power Assay.
The FRAP assay is high-throughput, adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents.
FRAP assay principle
The assay measures the antioxidant potential in samples through the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the samples. Following the reduction of the ferric iron, a blue color develops that is read colorimetrically at 594 nm.
The antioxidant potential of samples is determined using a ferrous iron standard curve and results are expressed as Fe2+ equivalents (μM) or FRAP value.
FRAP assay protocol summary
- add samples and standards to wells
- add reaction mix
- measure absorbance at 594 nm in kinetic mode for 60 min at 37°C
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
