Description
Key facts
Detection method:Fluorescent,
Sample types:Adherent cells, Suspension cells,
Assay Platform:Microplate reader
Product details:
Cell Migration/Chemotaxis Assay Kit (96-well, 5 μm) (ab235693) utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. The assay is easy to use, sensitive and adaptable to high-throughput systems.
Cell migration / chemotaxis assay protocol summary
- Grow cells.
- Add cell culture media containing desired chemoattractant to the bottom part of the Boyden chamber (bottom chamber).
- Add cell culture media containing positive control Migration Inducer to control wells in the bottom chamber.
- Add cells to the top part of the Boyden chamber (top chamber) with any desired treatment.
- Incubate at 37°C in cell culture incubator for 2-48 h.
- Cells will migrate through the polycarbonate membrane from the top chamber to the bottom chamber.
- Remove the top chamber and remove media and remaining cells.
- Centrifuge the bottom chamber and wash cells with Wash Buffer, repeat.
- Add Cell Dye / Cell Disassociation Solution to bottom chamber and incubate for 60-min in cell culture incubator.
- Analyze with fluorometric (Ex/Em 530/590 nm) plate reader.
- Prepare a separate standard curve with known numbers of cells in a 96-well plate with Cell Dye, incubate for 1-hr, and analyze with fluorometric (Ex/Em 530/590 nm) plate reader to allow quantitation.
Other notes
This product was previously called K907 Biovision EZCell™ Cell Migration/Chemotaxis Assay Kit (96-well, 5 μm). Biovision was acquired by Abcam in 2021.
Cell migration is a process by which cells move from one location to another. Cell motility is observed in unicellular organisms, and is essential for the development and maintenance of multicellular organisms. Cells often migrate in response to specific external stimuli, including chemical & mechanical signals. Errors during this process have serious consequences, including intellectual disability, vascular disease, tumor formation and metastasis.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
