Description
Key facts
Detection method:Colorimetric,
Sample types:Adherent cells,
Assay type:Cell-based (quantitative),
Assay Platform:Microplate reader
Product details:
SRB Assay / Sulforhodamine B Assay Kit ab235935 is used as a cell viability assay.
SRB assay principle
The SRB Assay / Sulforhodamine B Assay relies on the stoichiometric binding of SRB (Sulforhodamine B) dye to proteins under mild acidic conditions and its subsequent extraction under basic conditions. The amount of dye extracted is a proxy for cell mass and thus the number of cells in a sample.
The measurement of the number of cells in the assay allows its use for analysis of cell viability, cytotoxicity and cell proliferation.
In the assay, cultured cells are fixed on plates, stained with Sulforhodamine B, washed and dried, then the bound dye is solubilized and the absorbance of the dye in solution is measured at OD 565 nm.
Review our cell health assay guide to learn about
cell viability assays and cytotoxicity assays
apoptosis assays
This product is manufactured by BioVision, an Abcam company and was previously called K943 Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric). K943-1000 is the same size as the 1000 test size of ab235935.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sulforhodamine B often referred to as SRB is a dye commonly used in biological assays. It has a mass of approximately 580.7 g/mol. Researchers extensively use SRB in cell proliferation and cytotoxicity assays due to its ability to bind to protein components. It is not naturally expressed in biological organisms since it is a synthetic compound. Instead scientists introduce it into cell cultures and tissues for experimental purposes to measure cellular protein content.
Biological function summary
The compound plays an essential role as a cell stain that helps in visualizing and quantifying cell growth. It binds to basic amino acid residues of proteins in cells allowing consistent measurement of cell density. SRB does not function as part of any molecular complex as its utility lies mainly in providing a colorimetric means for analyzing cell growth and viability.
Pathways
SRB serves as a significant tool rather than a participant in cellular pathways. It helps researchers assess cell metabolic pathways by measuring changes in protein synthesis. Although not part of the pathways it indirectly interacts with pathways such as apoptosis and cell cycle regulation through its usage in assays. SRB when bound to proteins aids in the evaluation of complex interactions within the cell helping scientists to infer the activity of other proteins involved in these pathways.
SRB aids in the research of cancer and other proliferative diseases. It is instrumental in drug screening assays to evaluate the effects of anti-cancer drugs on cell growth and survival. Although not directly linked to diseases its role in determining cell viability post-treatment assists researchers in understanding how drugs interact with proteins like p53 and caspases which are important in cancer pathogenesis and cell death processes.
