Description
Key facts
Purity:>85% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P17947,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PU.1 also known as Spi1 is a transcription factor that belongs to the ETS family of transcription factors. It plays a major role in gene regulation and is an important player in hematopoietic cell differentiation. PU.1 has a molecular mass of approximately 31 kDa and is predominantly expressed in hematopoietic cells such as myeloid and B-lymphoid cells. It actively interacts with specific DNA sequences to regulate the expression of target genes.
Biological function summary
PU.1 influences the development and function of various blood cells including macrophages neutrophils and B-cells. It is essential for the regulation of genes involved in immune responses cell proliferation and survival. PU.1 functions as part of a larger transcriptional regulatory complex and often partners with other transcription factors and coactivators to exert its effects. This cooperation allows precise gene expression control in specific cell lineages.
Pathways
Expression of PU.1 is critical in the hematopoietic development pathway and the immune response pathway. It closely interacts with other transcription factors like GATA-1 and C/EBPα forming a network that affects hematopoietic lineage commitment. Within these pathways PU.1 constantly coordinates signals that influence progenitor cell fate and differentiation ensuring a balanced proportion of cell types within the blood.
PU.1 has significant implications in leukemia and other hematological malignancies. Dysregulation or mutation of PU.1 can lead to improper hematopoietic cell development and contribute to acute myeloid leukemia (AML) and other blood disorders. Additionally its expression level is closely linked with immune system function associating PU.1 indirectly with autoimmune conditions. In these contexts the interaction with other proteins like AML1 and C/EBPγ can influence disease pathogenesis by modulating gene expression patterns critical for normal hematopoietic and immune function.
