Description
Product details:
HRP Antibody Conjugation Check Kit (ab236555) is a quick immunochromatography test that allows to confirm the successful conjugation of Horseradish Peroxidase (HRP) to an antibody without the need for any specialized or costly equipment.
The key component of the kit is a nitrocellulose membrane containing a Test line of immobilized Protein A and Protein G called a half strip. Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules. The half strips also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. The HRP-antibody conjugate is run on the Protein A/G Strip. The conjugate binds the Protein A and Protein G concentrated on the Test line. After the addition of the HRP detection solution, a visible line appears on the strip.
Benefits:
Quick and easy qualitative test to check success of conjugation
Sensitivity: the range of detection is between 0.5 ng/mL to 10 ng/mL.
Compatible with antibody conjugates generated with Lightning-Link® HRP conjugation kit (ab102890) or any other HRP conjugation technologies available commercially
No costly equipment required
Small sample volumes required (40 μL)
This product is manufactured by Expedeon, an Abcam company. It was previously called HRP Check&Go! Antibody Labeling Confirmation Kit. Expedeon product code 4002-0030 is the same as the 30-test size of this product.
Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
