Description
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Plasma, Serum,
Assay time:1h 20m,
Assay Platform:Microplate reader
Product details:
Bile Acid Assay Kit (Colorimetric) ab239702 provides a simple, sensitive, and high-throughput suitable approach to quantify the concentration of total bile acid in biological fluids.
The bile acid assay is based on an enzymatic cycling method in the presence of NADH and a chromophore. The reduction of the chromophore produces a stable colorimetric product the absorbance of which can be followed kinetically at 405 nm on a multi-well spectrophotometer. This absorbance is directly proportional to the amount of total bile acids in the sample.
Other metabolites found in biofluids do not interfere with the assay.
The assay can detect as little as 1 μM of Bile Acids in a variety of samples.
Bile acid assay protocol summary:
- add samples and standards to wells
- add probe mix and incubate at 37°C for 10 min
- add reaction mix
- measure absorbance at 405 nm for 60 min at 37°C in a kinetic mode
This product is manufactured by BioVision, an Abcam company and was previously called K209 Total Bile Acids (TBA) Assay Kit (Colorimetric). K209-100 is the same size as the 100 test size of ab239702.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bile acids also known as bile salts are amphipathic molecules synthesized in the liver. These molecules with a mass of around 408 to 465 Daltons depending on their specific form are stored in the gallbladder and released into the small intestine. They play a mechanical role in emulsifying dietary fats which assists in the absorption of fat-soluble vitamins and nutrients. Bile acids are mainly expressed in the liver where their biosynthesis occurs and they can also be reabsorbed in the ileum and returned to the liver via enterohepatic circulation.
Biological function summary
Bile acids facilitate the digestion process by breaking down large fat globules into smaller emulsified micelles. This activity enables lipase to effectively catalyze triglyceride hydrolysis. Bile acids are also part of the lipid transport complex and help maintain cholesterol homeostasis. Aside from their role in lipid absorption bile acids act as signaling molecules influencing metabolic processes by binding to receptors such as the farnesoid X receptor (FXR) and G-protein-coupled bile acid receptor 1 (GPBAR1 or TGR5).
Pathways
Bile acids participate significantly in the digestive and hepatic pathways. They interact with nuclear receptors like FXR to regulate the expression of specific proteins involved in bile acid synthesis and transport such as cytochrome P450s (CYP7A1). Through these pathways bile acids help maintain energy homeostasis and glucose metabolism influencing proteins like fibroblast growth factor 19 (FGF19) that modulate hepatic bile acid synthesis and fatty acid metabolism.
Bile acids have connections with conditions like cholestasis and gallstone formation. Cholestasis results from impaired bile flow leading to an accumulation of bile acids that may cause liver damage. Bile acids are also linked to metabolic syndromes as they affect enterohepatic signaling pathways. The nuclear receptors FXR and TGR5 associated with bile acid action serve as targets for therapeutic interventions in managing these disorders by modulating bile acid metabolism and signaling pathways.
