Description
Key facts
Detection method:Fluorescent,
Sample types:Tissue, Cell culture extracts, Cell culture media
Product details:
Soluble Collagen Assay Kit (ab241015) detects newly synthesized (acid-soluble) collagen levels in various biological samples and cultured cell medium.
The assay involves extraction of soluble collagen in acetic acid, followed by enzymatic degradation of collagen into glycine-rich oligopeptides, which are quantified using a fluorogenic reagent and developer solution that selectively react with the N-terminal glycine fragments to form a stable fluorescent complex (Ex/Em = 376/468 nm).
The assay is more sensitive and selective than dye-binding (Picrosirius Red) soluble collagen assays, is simple to perform and has a linear range from 0.05 - 2 ug collagen per well (2.5 ug/mL to 100 ug/mL in a 20 uL sample volume).
Other Notes
This product was previously called K532 Soluble Collagen Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagen also known as collagen peptide is a protein that gives structural support to connective tissues. It is the most abundant protein in mammals with collagen affecting the structure and resilience of tissues such as skin tendons cartilage and bones. Collagen molecules consist of triple helical structures and depending on the type their molecular weights vary significantly generally around 300 kDa. Fibroblasts primarily express collagen and it also exists in other cell types in smaller amounts. Its role in forming fibrous structures gives tissues their mechanical strength and elasticity.
Biological function summary
Collagen participates in the formation of extracellular matrices by organizing fibers into supramolecular structures. These fibers provide structural integrity and cell anchoring in tissues. Collagen interacts with proteins like elastin and proteoglycans forming a network important for tissue elasticity. It forms a part of the greater collagen complex working with various collagen types such as types I II and III which contribute to tissue-specific functionalities. Its presence influences processes like wound healing and tissue remodeling and collagen testing helps analyze its structural state in tissues.
Pathways
Collagen integrates into signaling pathways that involve tissue repair and development particularly the TGF-beta pathway. This pathway plays a role in fibrotic responses and regulates collagen expression and deposition. Collagen also associates with proteins like matrix metalloproteinases which regulate extracellular matrix remodeling by cleaving collagen fibers. Pathways involving integrins further connect collagen to cell adhesion and migration processes emphasizing its role in cell interaction within the tissue matrix.
Collagen imbalances relate to diseases such as osteogenesis imperfecta and Ehlers-Danlos syndrome where faulty collagen synthesis or structure leads to weakened connective tissues. Osteogenesis imperfecta connects to mutated collagen type I or related genes impairing bone formation. Ehlers-Danlos syndrome involves disorders in collagen synthesis and can link to defective pro-collagen enzymes. In fibrotic conditions excessive collagen accumulates and disrupts normal tissue function affecting organs like the liver and lungs. Collagen assays such as the hydroxyproline assay measure collagen levels in biological samples supporting diagnostic evaluations.
