Description
Key facts
Detection method:Fluorescent,
Sample types:Cell Lysate
Product details:
ab242293 measures diacylglycerol (DAG) content in samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG samples, yielding phosphatidic acid. Next, a lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Finally, the glycerol-3- phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide which reacts with the kit's Fluorometric Probe (Ex. 530-560 nm/Em. 585-595 nm).
ab242293 is a simple, fluorometric assay that quantitatively measures total DAG in cell lysates using a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. The kit contains a DAG Standard and has a detection sensitivity limit of ~15 uM.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Diacylglycerol (DAG) is a lipid molecule that plays a mechanical role as a secondary messenger in cellular processes. It is sometimes referred to as DAG lipid or diacylglycerols. DAG consists of two fatty acid chains bonded to a glycerol backbone. DAG is expressed in the cell membrane and cytoplasm across various tissues. The mass of this molecule varies depending on the length and saturation of its fatty acid chains which can impact its specific role and function in different biological contexts.
Biological function summary
Diacylglycerol participates in signal transduction pathways by activating protein kinase C (PKC) an important component in cellular signaling. DAG does not typically function alone; it often acts within multiprotein complexes influencing various cellular responses through modulation of PKC activity. Through these interactions DAG regulates processes like cell growth differentiation and apoptosis.
Pathways
DAG is a central component in the phosphoinositide pathway and the mitogen-activated protein kinase (MAPK) pathway. In the phosphoinositide pathway it works alongside inositol trisphosphate (IP3) to transduce extracellular signals into cellular responses. In the MAPK pathway DAG aids in the activation of proteins that play roles in proliferation and stress response. Related proteins include phospholipase C which produces DAG by hydrolyzing phosphatidylinositol 45-bisphosphate and PKC which is activated by DAG to propagate intracellular signaling.
DAG is linked to conditions such as cancer and obesity. Dysregulation of DAG levels can lead to uncontrolled cell proliferation contributing to tumorigenesis. In obesity altered DAG signaling may impair insulin sensitivity affecting glucose metabolism. In these contexts the protein kinase C family is often implicated due to DAG's role in PKC activation making DAG a target of interest in disease modulation and treatment strategies.
