Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.2 pg/mL,
Range:6.25 - 400 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human 4-1BBL ELISA Kit ab246536 is a rapid single-wash 90-min ELISA kit to measure Human 4-1 BBL in Citrate plasma, Cell culture supernatant, Serum. This SimpleStep ELISA® sensitivity is 5.64 pg/mL.
How the assay works
Human 4-1 BBL SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of Human 4-1BBL. The standard protein in this kit is the extracellular domain of Human 4-1BBL. Native signal was detected in cell culture supernatant. Spiked protein experiments were used to validate serum and plasma (citrate) sample types. Neat pooled serum and plasma (citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay. Saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit. This kit is incompatible with plasma (EDTA) and plasma (heparin) samples.
Human 4-1BBL ELISA Kit ab246536 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
Save your precious samples
Our 384-well Human 4-1BBL ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human 4-1BBL Antibody Pair - BSA and Azide free
ab256681
- Anti-4-1BBL antibody [EPR21995-69] - BSA and Azide free (Capture)
ab259609
- Anti-4-1BBL antibody [EPR21995-47] - BSA and Azide free (Detector)
ab259610
Related and recommended products
Human 4-1BBL ELISA Kit ab246536 is commonly used to study immune cell activation and regulation:
Other kits for measuring immune response include:
- Human CD40L ELISA kit
ab99991
- Human TNFSF4 ELISA kit (OX40L)
ab213842
- Human TNF alpha ELISA Kit
ab181421
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
