Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-MAPK3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ERK1 also known as MAPK3 is an extracellular signal-regulated kinase involved in transmitting signals from the cell surface to the nucleus. This protein has a molecular mass of about 44 kDa. ERK1 expresses in various tissue types with higher expression in the brain heart and skeletal muscle. Researchers often study ERK1 in the context of its role in cellular signaling due to its involvement in critical regulatory functions.
Biological function summary
ERK1 plays a significant role in cell cycle regulation differentiation and proliferation. It forms part of the MAPK signaling cascade becoming activated through a phosphorylation event. In its activated form ERK1 translocates to the nucleus where it phosphorylates target substrates. ERK1 often functions in conjunction with its homolog ERK2 to mediate these cellular processes marking it as an essential player in growth factor signaling.
Pathways
ERK1 functions primarily within the MAPK/ERK signaling pathway a major conduit for transmitting proliferative signals from growth factor receptors. ERK1 interacts with proteins like MEK1/2 which phosphorylate and activate ERK1 in response to extracellular stimuli. Another critical pathway involving ERK1 is the Ras-Raf-MEK-ERK cascade which regulates various cellular outcomes. This connection to the Ras family highlights its importance in signal transduction and reinforces its position in critical cellular processes.
Aberrant activation of ERK1 connects to diseases such as cancer and cardiovascular disorders. In cancer the dysregulation of the MAPK/ERK pathway often through mutations affecting Ras or Raf proteins leads to uncontrolled cell proliferation. ERK1's involvement in cardiovascular diseases links to its role in hypertrophic signaling in cardiac cells where altered ERK1 activity can contribute to pathological cardiac remodeling. Understanding these interactions can aid in developing therapeutic strategies targeting the MAPK/ERK signaling pathway.
