Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-BCL10, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bcl10 also known as B-cell lymphoma 10 is a protein involved in several cellular processes. It has a molecular mass of approximately 26 kDa and shows expression in various lymphoid tissues. Bcl10 plays a significant role in signaling pathways by transmitting signals from the cell surface to the nucleus impacting cell growth and immune responses. Researchers often study Bcl10 using immunohistochemistry (IHC) techniques referred to as Bcl10 IHC to analyze its expression and distribution within tissues.
Biological function summary
Bcl10 activates downstream signaling pathways that are critical for immune cell functioning and survival. It forms part of the CARD-BCL10-MALT1 (CBM) complex which is important for the activation of the NF-kB pathway. This complex formation is essential for lymphocyte activation and proliferation. Bcl10 interactions within this complex influence cellular responses to various stimuli helping organisms to mount appropriate immune responses.
Pathways
Bcl10 serves as an integral component in the NF-kB and TCR signaling pathways. It functions alongside proteins such as MALT1 and CARD9 in these pathways. The proper functioning of Bcl10 in these pathways ensures accurate transcriptional regulation necessary for immune responses. Misregulation in these pathways can lead to immune disorders and aberrant cell behavior linking Bcl10 to further biological implications.
Bcl10 shows associations with mucosa-associated lymphoid tissue (MALT) lymphoma and other inflammatory diseases. Aberrant Bcl10 expression or mutations can lead to unchecked activation of NF-kB contributing to the progression of these conditions. In MALT lymphoma Bcl10 often cooperates with MALT1 both contributing to pathological cell survival and proliferation highlighting the importance of Bcl10 in understanding these diseases.
