Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-COIL, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Coilin also known as Coilin protein or its gene name COIL is a nuclear phosphoprotein with a mass of approximately 62 kDa. It plays a mechanical role in the formation and maintenance of Cajal bodies which are subnuclear structures involved in various RNA processes. Coilin is mainly expressed in the nucleus of eukaryotic cells including a high expression in HeLa cells where its localization to Cajal bodies can be easily studied with tools like Alexa Fluor 488. Occasionally references may be made to terms like 'HeLa coil' which pertain to specific structural features noted in these contexts.
Biological function summary
This protein facilitates the organization and biogenesis of nuclear bodies associated with RNA processing and small nuclear ribonucleoproteins (snRNPs) assembly. Coilin operates as part of a larger complex involving several other proteins that interact within Cajal bodies. These nuclear bodies serve as sites where components necessary for RNA processing are concentrated. Coilin aids in the trafficking and modification of snRNP elements which are essential for pre-mRNA splicing.
Pathways
Coilin affects RNA metabolism and processing notably within the spliceosome assembly pathway and in snRNP biogenesis. The pathways include interactions with proteins such as SMN (Survival of Motor Neuron) protein which links to spinal muscular atrophy-related processes. Additionally Coilin contributes to the broader framework of nucleic acid processing systems impacting the outcome of RNA transcription and modification through its regulatory roles.
Coilin shows connections to neurological conditions and cancer. For instance alterations or dysregulation in Coilin expression can correlate with spinal muscular atrophy where it associates with the SMN protein if there are disruptions in Cajal body integrity. Furthermore links exist with certain cancers where the mismanagement of RNA processes influenced by Coilin and its interaction partners like PDEdelta can lead to aberrant cell behaviors and tumor progression. Coilin’s involvement in such diseases makes it an appealing target for therapeutic exploration reflected in ongoing cancer research and therapeutic developments.
