Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-FERMT2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Kindlin-2 also known as FERMT2 is a mechanistic component involved in cellular adhesion processes. This protein has a molecular weight of approximately 75 kDa. Kindlin-2 is found in various tissues with high expression in epithelial cells and muscle tissues. It works together with integrins to maintain cell adhesion to the extracellular matrix performing an important role in cell communication with its surroundings.
Biological function summary
Kindlin-2 contributes to integrin activation and cytoskeletal organization by forming part of a larger complex. This complex stabilizes integrin-talin interactions enhancing the affinity of integrins for their ligands. This function of Kindlin-2 aids in dynamically regulating cell shape migration and mechanical signaling. Its role is significant for normal cellular function and homeostasis because it helps cells to attach and detach when necessary which is important for cell movement and tissue repair.
Pathways
The action of Kindlin-2 integrates with key signaling pathways like the Wnt and PI3K/AKT pathways. In these pathways Kindlin-2 assists in transmitting signals necessary for cellular proliferation and survival. It interacts with other proteins such as talin and integrin β subunits forming a bridge between the cytoskeleton and external signals. This interaction allows cells to adapt to changes in their environment by modulating cell adhesion and migration.
Kindlin-2's function relates to conditions such as cancer and fibrosis. In cancer Kindlin-2 aids tumor cell migration and metastasis linking it to the pathological spread of cancerous cells. In fibrosis Kindlin-2 influences the excessive deposition of extracellular matrix components contributing to tissue scarring. The protein also interacts with fibronectin and collagens in these contexts showing its involvement in extracellular matrix remodeling and pathological tissue changes.
