Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GSTK1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GSTK1 also known as Glutathione S-transferase kappa 1 is an enzyme with a mass of approximately 26 kDa. This protein belongs to the glutathione S-transferase family which plays a significant role in detoxification processes through conjugation reactions. GSTK1 displays a broad tissue expression with abundant presence in liver and kidneys as well as other tissues which facilitates its involvement in protecting cells from oxidative stress and chemical damage.
Biological function summary
GSTK1 is part of the cellular defense mechanism against xenobiotic and oxidative stress. This enzyme participates in the neutralization of harmful compounds by facilitating their conjugation with glutathione a powerful antioxidant. GSTK1 functions independently and does not form part of a larger protein complex. Its activity is important in maintaining cellular homeostasis and preventing damage caused by reactive oxygen species.
Pathways
GSTK1 has critical involvement in glutathione metabolism and xenobiotic detoxification pathways. It interacts functionally with proteins like GSTM1 which are also part of the glutathione S-transferase family enhancing the cell’s ability to detoxify harmful substances. GSTK1 helps to modulate the cellular response to oxidative stress through these pathways ensuring protection against toxicity.
GSTK1 has been linked to conditions including cancer and liver diseases. Its detoxification role makes GSTK1 significant in cancer biology where its expression levels may influence tumor resistance to chemotherapy. Additionally GSTK1 has connections with other glutathione S-transferases like GSTP1 which have associations with similar disorders affecting cellular protection mechanisms.
