Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 2 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-IRF1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IRF1 also known as Interferon Regulatory Factor 1 is a transcription factor playing an important role in the regulation of interferon genes. This protein has a molecular weight of approximately 37 kDa. IRF1 is most commonly expressed in the nucleus but can be found elsewhere in the cell depending on the context. It operates by binding specific DNA sequences and influencing the transcription of genes involved in immune response.
Biological function summary
IRF1 influences cell growth arrest apoptosis and immune responses. It acts as an important regulator in the transcription of interferon-alpha and beta genes. IRF1 functions independently and is not generally part of a larger complex although it interacts with other transcription factors to modulate gene expression.
Pathways
IRF1 plays a significant role in both the type I interferon signaling pathway and the NF-kB pathway. These pathways are essential in the body's response to viral infections and inflammation. IRF1 interacts with proteins like STAT1 and NF-kB to initiate and regulate these immune responses effectively coordinating cellular reactions to external stress signals.
IRF1 has associations with cancer and autoimmune diseases. In several cancer types such as gastric cancer IRF1 expression levels can affect tumor suppression and progression. IRF1 also interacts with proteins like P53 in the context of cancer highlighting its role in immune and apoptotic pathways. In autoimmune conditions like multiple sclerosis altered IRF1 activity can impact disease severity and progression often through its interactions with other immune mediators.
