Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-LMAN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LMAN1 also known as ERGIC-53 or lectin mannose-binding 1 is a protein involved in the transport and quality control of glycoproteins. It has a mass of approximately 53 kDa. LMAN1 primarily expresses in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and is important for efficient cargo sorting and proper folding. It functions by binding to specific carbohydrate moieties on glycoproteins aiding their transport between the ER and Golgi apparatus.
Biological function summary
LMAN1 functions significantly within a protein complex known as the LMAN1-MCFD2 complex. It serves an essential role as a cargo receptor for glycoproteins particularly those with particular mannose-rich domains influencing their secretion and stability. This protein complex is integral in maintaining protein homeostasis and cellular physiology by ensuring the correct sorting and transport of glycoproteins from the ER to the Golgi.
Pathways
LMAN1 significantly participates in the secretory pathway and quality control mechanisms in the cell. It interacts with proteins such as MCFD2 to mediate the transport of specific glycoproteins like α1-antitrypsin through the secretory pathway. This pathway is important for the secretion of a variety of proteins and disruptions can lead to disorders associated with improper protein folding and trafficking.
Defects in LMAN1 can lead to bleeding disorders such as combined coagulation factor V and VIII deficiency. The protein's dysfunction impairs the transport of coagulation factors disrupting their secretion. Additionally proteins like MCFD2 work closely with LMAN1 in this context as mutations in either protein can cause a similar deficiency highlighting the significant role of LMAN1 in maintaining normal hemostatic processes.
