Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7 and 2 bp deletion in exon7.,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-MAP3K2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MEKK2 also known as MAP3K2 is a kinase enzyme with an approximate mass of 70 kDa. It functions as part of the MAP kinase signaling pathway where it phosphorylates downstream proteins playing an essential role in transmitting signals within cells. This protein is mainly expressed in tissues such as the brain heart and liver. It acts by activating specific MAP kinase pathways facilitating various cellular responses to external stimuli.
Biological function summary
This kinase serves as a critical mediator in cellular processes like proliferation differentiation and apoptosis. MEKK2 forms part of larger signaling complexes including the MAP3K complex where it regulates multiple cellular activities. Through its kinase activity MEKK2 passes on signals that affect gene expression and cellular stress responses impacting how cells react to their environment.
Pathways
MEKK2 functions prominently in the MAPK signaling and NF-kB pathways. These pathways involve signal transduction mechanisms that MEKK2 influences through its interactions with related proteins such as MEK5 and ERK5. By modulating these pathways MEKK2 participates in a variety of cellular responses including inflammation and immune responses.
MEKK2 has associations with conditions like cancer and inflammatory diseases. Aberrant expression of MEKK2 can affect the behavior of cancer cells due to its influence on the MAPK pathway's signaling leading to unchecked cell growth. Additionally MEKK2 interacts with proteins like NF-kB which are important in inflammation thereby implicating it in disorders related to immune system dysfunctions.
