Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-S100A11, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
S100A11 also known as Calgizzarin or MLN 70 is a calcium-binding protein of the S100 family. It has a molecular mass of approximately 11 kDa. S100A11 can be found in a wide range of tissues including skin placenta and heart. It exhibits the ability to bind calcium ions through its EF-hand motifs which influences its activity and stability. These calcium-binding domains make S100A11 an important regulator of various intracellular processes by responding to changes in calcium ion concentrations.
Biological function summary
The functions of S100A11 include involvement in cell proliferation differentiation and migration. S100A11 often operates as part of a protein complex facilitating these cellular processes by interacting with other signaling molecules. It can shuttle between the cytoplasm and nucleus influencing gene expression and cellular dynamics. S100A11 has also shown involvement in smooth muscle contraction and skin barrier function regulation.
Pathways
The activity involving S100A11 spans multiple biological pathways including the calcium signaling pathway and the epithelial cell signaling pathway. Through these pathways S100A11 maintains critical cellular homeostasis and function. In the calcium signaling pathway it interacts with proteins like annexins and calcyclin to modulate cellular responses to calcium influx. In epithelial cell signaling it affects pathways related to cell adhesion and communication integrating signals that dictate cell behavior in response to external stimuli.
S100A11 is relevant to cancer progression and inflammatory skin disorders. Its overexpression in various cancers such as bladder and prostate cancer suggests a role in tumor growth and metastasis. During the cancerous process S100A11 interacts with the p53 protein which impacts cell cycle regulation and survival. Additionally in inflammatory skin conditions like psoriasis S100A11 influences keratinocyte activity contributing to abnormal skin scaling and inflammation through interactions with filaggrin and keratin proteins.
