Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 7 bp deletion in exon2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-AZI2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AZI2 also known as Nak-associated protein 1 (NAP1) is a regulatory protein with a molecular mass of about 55 kDa. Its primary role involves interaction with other molecules to help signal transduction in cells. AZI2 mainly express in tissues that are involved in immune responses such as the spleen and lymph nodes. It functions through modulating the activation of signaling pathways critical for cellular responses to various stimuli.
Biological function summary
AZI2 plays an essential role in the immune system. It is part of a signaling complex that includes other proteins such as TANK and TRAF6. This complex contributes to the activation of NF-kB transcription factors. AZI2 is involved in cellular processes like inflammation and response to stress by regulating the activity of NF-kB an important transcription factor. This regulatory activity underlines AZI2's significance in maintaining immune system homeostasis.
Pathways
AZI2 is closely associated with the toll-like receptor (TLR) and TNF receptor signaling pathways. These pathways orchestrate immune cell responses to microbial infections and inflammation. In these pathways AZI2 interacts with proteins like IKK epsilon and TANK to mediate NF-kB activation. These interactions help transduce signals from membrane-bound receptors to the nucleus promoting the expression of immune response genes.
Aberrant AZI2 function can link to autoimmune diseases and certain cancers. Disruption of AZI2-related signaling pathways can lead to improper immune system activation contributing to autoimmune diseases like rheumatoid arthritis. In cancer altered AZI2 expression can affect pathways that control cell survival and proliferation. Through these diseases AZI2 has associations with key regulatory proteins like TRAF3 and TBK1 which play roles in modulating immune responses and cellular growth.
