Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycerol kinase also known as GK or Gyk acts as a catalyst in the phosphorylation of glycerol to glycerol-3-phosphate. This enzymatic activity is important for the utilization of glycerol in metabolic processes. Glycerol kinase is expressed in tissues such as liver kidney and adipose tissue. Its molecular weight varies depending on the species but in humans it is around 60 kDa when analyzed using methods like glycerol SDS PAGE. In human cells like HEK293T reliable detection of glycerol kinase is possible due to its considerable expression.
Biological function summary
The main role of glycerol kinase relates to its function in lipid metabolism and energy homeostasis. It exists as a part of a biochemical pathway but does not typically form a complex with other proteins. Glycerol kinase enables cells to utilize glycerol as an energy source by converting it into glycerol-3-phosphate which feeds into further metabolic pathways. This conversion is essential for the balance of energy supply and the production of triglycerides and phospholipids.
Pathways
Metabolism and energy production heavily rely on the function of glycerol kinase. This enzyme acts within the glycerolipid metabolism and gluconeogenesis pathways. In these pathways it is connected to other enzymes like glycerol-3-phosphate dehydrogenase which further processes the glycerol-3-phosphate produced by glycerol kinase. The enzyme's availability affects the rate of these pathways and in turn influences lipid and glucose metabolism in the body.
Glycerol kinase deficiency leads to complex metabolic syndromes such as hyperglycerolemia and congenital adrenal hyperplasia. Hyperglycerolemia results from excess glycerol in the blood due to impaired conversion by the enzyme. In congenital adrenal hyperplasia alterations in metabolic function relate to glycerol kinase’s impact on stress response and energy balance. Disorders related to glycerol kinase sometimes involve interactions with other proteins and enzymes involved in broader metabolic processes affecting overall metabolism and physiology.
