Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 2 bp deletion in exon 3.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HNRNPR, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heterogeneous nuclear ribonucleoprotein R commonly known as hnRNP R is a multifunctional protein involved in mRNA processing. It is approximately 72 kDa in size and found mostly in the nucleus. hnRNP R exhibits a strong expression in neural tissues and various other cell types. As a member of the hnRNP family it plays an important role in RNA-binding activities and influences the splicing process export and stability of mRNA.
Biological function summary
HnRNP R contributes to several cellular tasks related to gene expression regulation. This protein interacts with other hnRNPs to form core components of ribonucleoprotein complexes. It participates in modulating RNA transport from the nucleus to the cytoplasm and facilitates mRNA translation. Additionally hnRNP R associates with other proteins and RNA molecules strengthening the cellular response to environmental cues.
Pathways
HnRNP R acts decisively in several gene expression and RNA processing pathways. It is involved in the mRNA splicing pathway where it interacts with splicing machinery to process precursor mRNAs into mature mRNAs. hnRNP R also influences the Wnt signaling pathway by regulating the stability and localization of mRNAs involved in this pathway. Moreover it interacts with proteins like hnRNP A1 which further aids its functional capabilities in these pathways.
HnRNP R associates with neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Its interaction with other hnRNPs like TDP-43 suggests a link to the RNA-binding protein misregulation observed in these disorders. Research shows that abnormalities in hnRNP R function can lead to widespread disruption of mRNA processing which contributes to the pathogenesis of neurodegenerative diseases.
