Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-NCK1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Nck protein also referred to as Nck1 n c k or Nck is an adaptor protein integral in cell signaling pathways. It consists of a molecular mass around 45 kDa and exists mainly within the cytoplasm. The Nck proteins including Nck1 and its closely related form Nck2 are particularly prominent in cells of the immune and nervous systems. Functionally they facilitate interactions between receptor tyrosine kinases and downstream signaling molecules translating extracellular stimuli into cellular responses.
Biological function summary
The Nck proteins play significant roles in actin cytoskeleton reorganization influencing cell shape movement and adhesion. These proteins often participate as part of larger signaling complexes where they serve as scaffolds bringing together key molecules. Nck proteins directly interact with partners containing SH2 and SH3 domains which are important for various cellular dynamic processes. Their role in cytoskeletal dynamics aligns them with many cellular mechanisms including endocytosis and cell motility.
Pathways
Nck proteins significantly influence the regulation of the actin cytoskeleton pathway and the Rho GTPases pathway. Through these pathways they interact with proteins such as WASP and Rac regulating actin polymerization and cell migration. Nck’s ability to form complex signaling networks positions it as a central actor in transducing signals that affect cell shape and movement. It acts collaboratively with multiple signaling pathways to maintain cellular plasticity and response to extracellular signals.
Nck proteins have associations with cancer and immunological conditions. Alterations in their signaling functions may contribute to tumor progression or metastasis given their role in cell movement and adhesion. In immune disorders abnormal Nck signaling could disrupt normal immune cell functions potentially leading to autoimmune conditions. For example Nck proteins may interact with oncogenic proteins like BCR-ABL in chronic myeloid leukemia highlighting their relevance in disease contexts.
