Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-NUDT5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Nudix hydrolase 5 (NUDT5) enzyme also known as ADP-sugar pyrophosphatase performs important mechanical roles by hydrolyzing ADP-ribose to ribose-5-phosphate and AMP. It holds a molecular mass of approximately 39 kDa. NUDT5 shows high expression levels in tissues like liver and ovary. Its activity involves detoxifying nucleoside diphosphate derivatives which prevents their accumulation and potential interference with normal cellular processes.
Biological function summary
NUDT5 influences intracellular signaling pathways and DNA repair processes. It operates as part of multi-enzyme complexes that manage nucleotide metabolism and signaling. By maintaining a balance of ADP-ribose inside cells NUDT5 helps regulate cellular stress responses and genomic stability. Its role in transforming ADP-ribose supports maintaining adequate cellular energy and nucleotide pools for various biological functions.
Pathways
Research identifies NUDT5 as being involved in the ADP-ribose metabolism pathway and DNA repair mechanisms. In the ADP-ribose pathway NUDT5 assists in the regulation of ADP-ribose levels working alongside other proteins like PARP1 which adds ADP-ribose units to proteins during DNA repair. NUDT5's actions in these pathways ensure the proper functioning of cellular stress responses and the prevention of genome instability.
NUDT5 has connections to tumorigenesis and neurodegenerative disorders. Elevated activity of NUDT5 is sometimes observed in certain cancers suggesting a potential role in cancer cell viability. In neurodegenerative diseases such as Parkinson's dysfunctional ADP-ribose metabolism implicates NUDT5. Changes in NUDT5 activity can interact with proteins like PARP1 altering cellular processes that contribute to disease pathogenesis.
