Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 5 bp deletion in exon4.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-PIP5K1C, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein PIP5K1C also known as phosphatidylinositol-4-phosphate 5-kinase type-1 gamma has a mass of approximately 75 kDa. This enzyme plays a critical role in the phosphatidylinositol signaling pathway where it catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PI4P) to produce phosphatidylinositol 45-bisphosphate (PIP2). PIP5K1C's activity is pivotal for membrane trafficking and it demonstrates widespread expression in tissues including the brain and skeletal muscle.
Biological function summary
PIP5K1C is involved in the regulation of actin cytoskeleton dynamics. It participates as an important enzyme in complexes that modulate actin polymerization and cell adhesion processes. Its function impacts cell motility and maintenance of cell shape contributing significantly to endocytosis and exocytosis. This function highlights the enzyme's role in intracellular signaling and adhesion-related activities.
Pathways
PIP5K1C interacts intricately within the phosphoinositide signaling pathway and impacts the regulation of focal adhesion. It influences other proteins like RAC1 an important regulator of actin polymerization and talin which is involved in linking integrins to the actin cytoskeleton. These interactions place PIP5K1C as an important contributor to the molecular mechanisms that control cell movement and attachment.
Mutations or dysfunctions in PIP5K1C have been associated with neurological disorders such as Charcot-Marie-Tooth disease type 4F and L-fucosidosis. Abnormalities in PIP5K1C expression or activity can disrupt cellular functions and lead to these conditions. In the context of Charcot-Marie-Tooth disease PIP5K1C may interact with dynamin 2 a protein involved in endocytic vesicle fission which further influences the peripheral nervous system pathology.
